dok2 mouse monoclonal antibody (Santa Cruz Biotechnology)
Structured Review

Dok2 Mouse Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dok+2/Dok-2+Antibody/pmc12456515-154-5-11
Average 93 stars, based on 23 article reviews
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1) Product Images from "Identification of anti-DOK2 antibodies in patients with autoimmune hepatitis via a human protein microarray"
Article Title: Identification of anti-DOK2 antibodies in patients with autoimmune hepatitis via a human protein microarray
Journal: Hepatology Communications
doi: 10.1097/HC9.0000000000000807
Figure Legend Snippet: Serum anti-DOK2 antibody levels in AIH patients following PSL treatment. (A) Comparison of serum anti-DOK2 antibody levels at onset and posttreatment among AIH patients who achieved biochemical remission (left). Comparison of serum anti-DOK2 antibody levels at onset and posttreatment among AIH patients who did not achieve biochemical remission (right). p-v alues were calculated via the Wilcoxon matched-pairs signed-rank test; p <0.05 was considered significant. (B) Relationship between serum anti-DOK2 antibody and IgG levels posttreatment in patients with AIH. (C) Relationships between serum anti-DOK2 antibody levels and serum AST levels posttreatment in AIH patients. (D) Relationships between serum anti-DOK2 antibody levels and serum ALT levels posttreatment in AIH patients. p - v alues were calculated via the Wilcoxon matched-pairs signed-rank test. Abbreviations: AIH, autoimmune hepatitis; AU, arbitrary units; DOK, docking protein; PSL, prednisolone.
Techniques Used: Comparison
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Incubation:Article Title: Alteration of platelet GPVI signaling in ST-elevation myocardial infarction patients demonstrated by a combination of proteomic, biochemical, and functional approaches Article Snippet: Following the electrophoresis, proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). .. The membranes were blocked in 5% BSA in TBS-T (20 mM Tris-HCl (pH 7.6), 150 mM NaCl and 0.1% Tween 20) overnight at 4 °C and incubated for 90 min at room temperature with the following primary antibodies: mouse-anti PLCɣ2 (sc-5283), rabbit-anti SLP-76 (sc-9062), rabbit-anti Article Title: Dok-1 and Dok-2 are negative regulators of lipopolysaccharide-induced signaling Article Snippet: .. For immunoblotting, immunoprecipitates or cleared cell lysates were separated by SDS-PAGE and transferred to PVDF membrane (Bio-Rad Laboratories), which was then incubated with antibodies to phospho-ERK (Thr202/Tyr204), phospho-p38 (Thr180/Tyr182), phospho–IκB-α (Cell Signaling), phospho-JNK (Thr183/Tyr185), ERK, IκB-α, Article Title: Dok-1 and Dok-2 are negative regulators of lipopolysaccharide-induced signaling Article Snippet: .. For immunoprecipitation, cell lysates were cleared and incubated with antibodies to mouse Dok-1 (A3) or Article Title: Dok-1 and Dok-2 are negative regulators of T cell receptor signaling. Article Snippet: CD4+ splenic T cells (2 3 107 per 0.2 ml) purified by magnetic field exclusion or 25-14 T cells (1 3 107 per 0.2 ml) were treated with biotinylated anti-CD3e mAbs (10 lg ml 1) followed by cross-linking with streptavidin (25 lg ml 1) at 37 C. These cells were solubilized with the lysis buffer (1% NP-40, 150 mM NaCl, 10 mM Tris at pH 7.4, 0.5 mM EDTA, 10 mM NaF, 1 mM sodium orthovanadate, 10 lM sodium molybdate, 2 lg ml 1 aprotinin, 1 mM benzamidine, 5 lg ml 1 chymostatin, 5 lg ml 1 leupeptin, 1 lg ml 1 pepstatin A and 0.2 mM phenylmethylsulphonylfluoride), and cleared lysates (whole cell lysates) were subjected to immunoblotting with antibodies to phospho-ZAP-70, phospho-LAT, phospho-Erk (Cell Signaling, Danvers, MA, USA), Erk2 (Santa Cruz, Santa Cruz, CA, USA) or FLAG-tag (Sigma). .. Otherwise, they were sequentially incubated with antibodies to TCRf (Santa Cruz), Dok-1 or Produced:Article Title: Alteration of platelet GPVI signaling in ST-elevation myocardial infarction patients demonstrated by a combination of proteomic, biochemical, and functional approaches Article Snippet: Following the electrophoresis, proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). .. The membranes were blocked in 5% BSA in TBS-T (20 mM Tris-HCl (pH 7.6), 150 mM NaCl and 0.1% Tween 20) overnight at 4 °C and incubated for 90 min at room temperature with the following primary antibodies: mouse-anti PLCɣ2 (sc-5283), rabbit-anti SLP-76 (sc-9062), rabbit-anti Western Blot:Article Title: Dok-1 and Dok-2 are negative regulators of lipopolysaccharide-induced signaling Article Snippet: .. For immunoblotting, immunoprecipitates or cleared cell lysates were separated by SDS-PAGE and transferred to PVDF membrane (Bio-Rad Laboratories), which was then incubated with antibodies to phospho-ERK (Thr202/Tyr204), phospho-p38 (Thr180/Tyr182), phospho–IκB-α (Cell Signaling), phospho-JNK (Thr183/Tyr185), ERK, IκB-α, Article Title: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth Article Snippet: .. Expression of specific proteins was revealed by immunoblotting with antibodies directed against Dok-1, Article Title: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth. Article Snippet: .. Expression of specific proteins was revealed by immunoblotting with antibodies directed against Dok-1, Article Title: Dok-1 and Dok-2 are negative regulators of T cell receptor signaling. Article Snippet: CD4+ splenic T cells (2 3 107 per 0.2 ml) purified by magnetic field exclusion or 25-14 T cells (1 3 107 per 0.2 ml) were treated with biotinylated anti-CD3e mAbs (10 lg ml 1) followed by cross-linking with streptavidin (25 lg ml 1) at 37 C. These cells were solubilized with the lysis buffer (1% NP-40, 150 mM NaCl, 10 mM Tris at pH 7.4, 0.5 mM EDTA, 10 mM NaF, 1 mM sodium orthovanadate, 10 lM sodium molybdate, 2 lg ml 1 aprotinin, 1 mM benzamidine, 5 lg ml 1 chymostatin, 5 lg ml 1 leupeptin, 1 lg ml 1 pepstatin A and 0.2 mM phenylmethylsulphonylfluoride), and cleared lysates (whole cell lysates) were subjected to immunoblotting with antibodies to phospho-ZAP-70, phospho-LAT, phospho-Erk (Cell Signaling, Danvers, MA, USA), Erk2 (Santa Cruz, Santa Cruz, CA, USA) or FLAG-tag (Sigma). .. Otherwise, they were sequentially incubated with antibodies to TCRf (Santa Cruz), Dok-1 or SDS Page:Article Title: Dok-1 and Dok-2 are negative regulators of lipopolysaccharide-induced signaling Article Snippet: .. For immunoblotting, immunoprecipitates or cleared cell lysates were separated by SDS-PAGE and transferred to PVDF membrane (Bio-Rad Laboratories), which was then incubated with antibodies to phospho-ERK (Thr202/Tyr204), phospho-p38 (Thr180/Tyr182), phospho–IκB-α (Cell Signaling), phospho-JNK (Thr183/Tyr185), ERK, IκB-α, Membrane:Article Title: Dok-1 and Dok-2 are negative regulators of lipopolysaccharide-induced signaling Article Snippet: .. For immunoblotting, immunoprecipitates or cleared cell lysates were separated by SDS-PAGE and transferred to PVDF membrane (Bio-Rad Laboratories), which was then incubated with antibodies to phospho-ERK (Thr202/Tyr204), phospho-p38 (Thr180/Tyr182), phospho–IκB-α (Cell Signaling), phospho-JNK (Thr183/Tyr185), ERK, IκB-α, Expressing:Article Title: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth Article Snippet: .. Expression of specific proteins was revealed by immunoblotting with antibodies directed against Dok-1, Article Title: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth. Article Snippet: .. Expression of specific proteins was revealed by immunoblotting with antibodies directed against Dok-1, Generated:Article Title: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth Article Snippet: .. Expression of specific proteins was revealed by immunoblotting with antibodies directed against Dok-1, Article Title: CD200R1-CD200 checkpoint inhibits phagocytosis differently from SIRPα-CD47 to suppress tumor growth. Article Snippet: .. Expression of specific proteins was revealed by immunoblotting with antibodies directed against Dok-1, Immunoprecipitation:Article Title: Dok-1 and Dok-2 are negative regulators of lipopolysaccharide-induced signaling Article Snippet: .. For immunoprecipitation, cell lysates were cleared and incubated with antibodies to mouse Dok-1 (A3) or |
